Ammonium Inhibits Performance of Rhodopseudomonas palustris in Cyanobacterial Substrate
Bing Feng 2,4
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School of Material Science and Engineering, Henan Institute of Technology, Xinxiang 453003, China
Key Laboratory of Algal Biology of Chinese Academy of Sciences, Institute of Hydrobiology, University of Chinese Academy of Sciences, Wuhan 430072, China
Department of Environmental Science, Egerton University, P. O. Box 536-20115, Egerton-Kenya
Jiangxi Academy of Environmental Sciences, Nanchang 330039, China
Submission date: 2020-03-11
Final revision date: 2020-05-03
Acceptance date: 2020-05-07
Online publication date: 2020-08-17
Publication date: 2020-10-05
Pol. J. Environ. Stud. 2021;30(1):385–392
Though it is feasible of Rhodopseudomonas palustris (R. palustris) stimulation in the cyanobacterial substrate, less is known about its performance under the high ammonium-nitrogen (NH4-N) circumstance. In the present study, the performance of grown R. palustris Strain PUF1 under an NH4-N gradient were investigated. Results showed that both the bacterial density and the pigment synthesis were severely inhibited at an NH4-N concentration of 6.0 g/L, while the ultrathin structure of survived PUF1 wasn’t obviously changed in comparison to NH4-N concentration ≤3.0 g/L. Noticeably, at an NH4-N concentration of 3.0 g/L PUF1s recovered its biosynthesis of pigments in a six-day acclimation period. Importantly, the PUF1s thrived in algal substrate under the NH4-N concentration ≤1.0 g/L with per mL algal substrate 8.96 to 9.88×108 colony formation unit (CFU) on day six. Moreover, it was more diverse of the bacterial consortia in the low NH4-N treatments (≤1.0 g/L) than that of NH4-N concentration 3.0-6.0 g/L. Additionally, the excess NH4-N reduced the sequestration of phosphorus by PUF1s from the algal substrate. Based on the above findings, an NH4-N threshold up to 1.0 g/L was recommended, it herein produces substantial R. palustris biomass and achieves efficient nutrient sequestration from the protein-rich cyanobacterial feedstock.