Assessment of Aging in Saccharomyces Cerevisiae Yeast Mutants Using Microscopy Techniques
A. Krzepiłko
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Department of Agricultural Sciences in Zamość, University of Life Science in Lublin, Szczebrzeska 102, 22-400 Zamość, Poland
Pol. J. Environ. Stud. 2009;18(3):399–404
Different microscopy techniques were used to assess the aging of Saccharomyces cerevisiae yeast cells in stationary culture. Live/Dead staining was found to be as effective as viability tests at determining viability of yeast cells. Cells of dismutase-deficient sod1 and sod2 yeast mutants age faster than wild-type strains. Changes in appearance and decreasing in diameter size of the aging cells was observed using the Nomarski technique. The results of the fluorescent staining with Redox Sensor Red and mBCl suggest a disturbance of redox homeostasis in the cells, while vacuole staining with CellTracker Blue CMAC indicates cytoplasmic vacuolization. Yeast cell staining as carried out in this study can be used as a fast preliminary test, making it possible to determine changes taking place in the cell during aging.