Biological Activity of Wild Isolates of Entomopathogenic Nematodes to Horse- Chestnut Leaf Miner (Cameraria ohridella)
Joanna Matuska-Łyżwa1, Wiesław Kaca2, Paulina Żarnowiec2
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1Department of Zoology and Biological Didactics, Institute of Biology,
2Department of Microbiology, Institute of Biology,
The Jan Kochanowski University, Świętokrzyska 15, 25-406 Kielce, Poland
Submission date: 2014-11-24
Final revision date: 2014-12-10
Acceptance date: 2014-12-11
Publication date: 2015-05-20
Pol. J. Environ. Stud. 2015;24(3):1181–1184
One of the pests of white chestnut (Aesculus hippocastanum L.) is a horse-chestnut leaf miner (Cameraria ohridella). Effective methods of reducing the population of this butterfly are still under investigation. One of them is the use of insecticidal nematodes that occur naturally in the environment. This paper presents the results of the susceptibility of horse-chestnut leaf miner larvae on entomopathogenic nematodes of the genus Steinernema. The experiment was conducted at a temperature of 20ºC and a dose initialized to 100 nematodes/insect. Applied nematodes were harvested from the greenery of Swietokrzyskie Province. The results of the analysis showed that the larvae Cameraria ohridella are susceptible to insecticide nematode invasion isolated from different sources. It was also shown that the efficiency of killing an insect by nematodes (extensiveness of invasion) is not correlated with the ability to reproduce or the viability of successive generations of insecticidal nematodes.