Biosorption of Sb(III) to Exopolymers from Cyanobacterium Synechocystis sp.: a Fluorescence and FTIR Study
Daoyong Zhang1, Xiangliang Pan2, Guijin Mu1
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1State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry,
Chinese Academy of Sciences, Guiyang, 550002, Guizhou, China
2State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography,
Urumqi, 830011, Xinjiang, China
Pol. J. Environ. Stud. 2012;21(5):1497–1503
Antimony (Sb) pollution in Sb mining areas has been of growing environmental concern. However, limited information is available on environmental behavior and biogeochemical process of Sb. In the present study, complexation of Sb(III) with extracellular polymeric substances (EPS) from cyanobacterium Synechocystis sp. was investigated using excitation-emission matrix (EEM) fluorescence spectroscopy. Two protein-like fluorescence peaks were identified in the EEM spectra of EPS. Fluorescence of both peaks was clearly quenched by Sb(III). The quenching constants (logKa) and the binding constants (logKb) for peaks A and B were in the range of 3.21-4.13 and 3.22-4.14, respectively. The interaction between EPS and Sb(III) is spontaneous and endothermic. The binding of Sb(III) to EPS is dominated by the hydrogen bonding and Van der waals forces. FTIR analysis showed that polysaccharides in EPS also participated in complexation EPS with Sb(III).