The influence of the presence of certain amino acids at different concentrations on the catabolic activity of the bacteria Desulfotomaculum ruminis was studied. Introduction of amino acids of the simple chain molecule in concentrations up to 10 g/dm3 in the Starkey media leads to a small decrease of the sulphate conversion degree. A more significant decrease in the reaction rate is observed for amino acids with branched side chains such as valine. Moreover, it was shown that except for the D-asparagine isomer, which is not assimilable by the bacteria Desulfotomaculum ruminis, the other amino acids are a source of assimilable nitrogen. Serine meets the demands of the minimum substrates and is a source of assimilable carbon and nitrogen in the process of bacteria proliferation.