Cellulose-Degrading Strains: their Screening and Application to Corn Straw in Low-Temperature Environments
Shuang Zhang 1  
,   Dexin Shan 1  
,   Xiao Liu 1  
,   Mengrui Sun 1  
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College of Resource and Environment, Northeast Agricultural University, Harbin, China
Dexin Shan   

Northeast Agricultural University, Northeast Agricultural University, College of Resource and Environment, 150030 Harbin, China
Submission date: 2017-10-14
Final revision date: 2017-10-23
Acceptance date: 2017-10-26
Online publication date: 2018-05-24
Publication date: 2018-05-30
Pol. J. Environ. Stud. 2018;27(5):2349–2355
Cellulose-degrading strains play an important role in cellulose degradation at low temperatures. In this study, three cellulose-degrading strains with high carboxymethyl cellulase (CMCase) activity were isolated from the soil in a low temperature environment of 10ºC. ZS-7 was Cladosporium sp, ZS-8 was Alternaria porri, and ZS-10 was Trichoderma harzianum, based on 16s rDNA gene sequence analysis. They were mixed, marked as ZS-M, and used for corn straw degradation at 15ºC in order to determine their application properties. ZS-7, ZS-8, ZS-10, and ZS-M efficiently degraded corn straw by 45.98%, 47.96%, 34.215%, and 33.56%, respectively, after 30 days of stationary culture. The dynamics of CMC, β-Glucosidase, and FPA during corn straw degradation were determined. The CMCase activity of ZS-7 and ZS-8 was 31.732 U/ml and 31.111 U/ml, respectively, but the enzyme activities and degradation of ZS-M were lower than the single strain because of metabolic effects that influenced the degradation process. The pH values of ZS-7, ZS-10, and ZS-M were particularly stable, changing from 7.08 to 7.77 during the full degradation cycle. ZS-7, ZS-8, and ZS-10 could be applied more broadly to degrade corn straw in cold regions.