Cellulose-Degrading Strains: their Screening and Application to Corn Straw in Low-Temperature Environments
More details
Hide details
College of Resource and Environment, Northeast Agricultural University, Harbin, China
Dexin Shan   

Northeast Agricultural University, Northeast Agricultural University, College of Resource and Environment, 150030 Harbin, China
Submission date: 2017-10-14
Final revision date: 2017-10-23
Acceptance date: 2017-10-26
Online publication date: 2018-05-24
Publication date: 2018-05-30
Pol. J. Environ. Stud. 2018;27(5):2349–2355
Cellulose-degrading strains play an important role in cellulose degradation at low temperatures. In this study, three cellulose-degrading strains with high carboxymethyl cellulase (CMCase) activity were isolated from the soil in a low temperature environment of 10ºC. ZS-7 was Cladosporium sp, ZS-8 was Alternaria porri, and ZS-10 was Trichoderma harzianum, based on 16s rDNA gene sequence analysis. They were mixed, marked as ZS-M, and used for corn straw degradation at 15ºC in order to determine their application properties. ZS-7, ZS-8, ZS-10, and ZS-M efficiently degraded corn straw by 45.98%, 47.96%, 34.215%, and 33.56%, respectively, after 30 days of stationary culture. The dynamics of CMC, β-Glucosidase, and FPA during corn straw degradation were determined. The CMCase activity of ZS-7 and ZS-8 was 31.732 U/ml and 31.111 U/ml, respectively, but the enzyme activities and degradation of ZS-M were lower than the single strain because of metabolic effects that influenced the degradation process. The pH values of ZS-7, ZS-10, and ZS-M were particularly stable, changing from 7.08 to 7.77 during the full degradation cycle. ZS-7, ZS-8, and ZS-10 could be applied more broadly to degrade corn straw in cold regions.