The aim of the study was to determine the effects of lead on some growth parameters and chemical contents of soybean (Glycine max L.) seedlings. The study was conducted according to the factorial trial in a completely randomized experimental design with six replications. To investigate lead effects on nutrient uptake and metabolism, two soybean varieties were grown under aeroponic conditions and stressed with lead sulphate (PbSO₄) at five concentrations in a fully controlled aeroponic climatic cabin. According to the results, Fe, Cu, and Pb contents of the roots and leaves were increased in direct proportions with increasing lead concentrations, and the highest increase was seen in the groups exposed to the highest level of lead. By contrast, total amounts and concentrations of Na in the root, and accumulation of P, Ca, Na, Cd, and Ni in the leaves were reduced. Lead, which was accumulated in a dose-dependent manner in both soybean varieties, reduce the growth of the plant and cause lower uptake of some mineral by plant organs.