Effect of Enterobacter sp. CS2 and EDTA on the Phytoremediation of Ni-contaminated Soil by Impatiens balsamina
Nasim Ahmad Yasin 1  
,   Waheed Ullah Khan 2  
,   Sajid Rashid Ahmad 2  
,   Aamir Ali 3  
,   Aqeel Ahmad 1, 4  
,   Waheed Akram 1, 4  
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University of the Punjab, Lahore Pakistan
College of Earth and Environmental Sciences, University of the Punjab, Lahore Pakistan
University of the Sargodha, Sargodha Pakistan
College of Plant Sciences and Technology, Huazhong Agricultural University, Wuhan China
Waheed Ullah Khan   

University of the Punjab, New Campus Lahore, Pakistan, 54590 Lahore, Pakistan
Submission date: 2017-05-18
Final revision date: 2017-07-30
Acceptance date: 2017-07-31
Online publication date: 2018-08-06
Publication date: 2018-11-20
Pol. J. Environ. Stud. 2019;28(1):425–433
During our current study we evaluated the effect of ethylenediaminetetracetic acid (EDTA) and Enterobacter sp. CS2 on nickel stress alleviation and phytoextraction by Impatiens balsamina L in spiked soil. Nickel resistant Enterobacter sp. CS2 was isolated from soil polluted by industrial effluents. The I. balsamina seeds primed with Enterobacter sp. CS2 were raised in EDTA-supplemented soil (10 mM) contaminated with 0, 100, 200, and 300 mg kg-1 Ni for 50 days. The effect of different treatments on plant growth attributes, nickel tolerance index, bioconcentration factor, and translocation factor were evaluated. The Ni stress reduced plant growth, carotenoids, and chlorophyll (chl) content. However, higher Ni uptake and proline contents were observed in plants growing in Ni-contaminated soils. The Enterobacter sp. CS2 inoculation further enhanced Ni uptake and proline contents in I. balsamina plants growing under Ni stress. The inoculated plants showed improved shoot length, root length, carotenoid content, chl ‘a’ and ‘b’ contents, root and shoot dry weight. The Ni tolerance index in Enterobacter sp. CS2-assisted plants was much higher compared to un-inoculated ones. The inoculated plants supplemented with EDTA enhanced 39%, 34%, and 30% Ni uptake in roots respectively under 100, 200, and 300 mg kg-1 of Ni treatment as compared with un-inoculated plants. The data regarding bioconcentration factor and translocation factor showed that Ni phytoextraction capability of I. balsamina plants was significantly enhanced with the supplementation of Enterobacter sp. CS2 and EDTA.