ORIGINAL RESEARCH
Effect of Enterobacter sp. CS2 and EDTA on the Phytoremediation of Ni-contaminated Soil by Impatiens balsamina
Nasim Ahmad Yasin 1  
,  
Waheed Ullah Khan 2  
,  
Sajid Rashid Ahmad 2  
,  
Aamir Ali 3  
,  
Aqeel Ahmad 1, 4  
,  
Waheed Akram 1, 4  
 
 
More details
Hide details
1
University of the Punjab, Lahore Pakistan
2
College of Earth and Environmental Sciences, University of the Punjab, Lahore Pakistan
3
University of the Sargodha, Sargodha Pakistan
4
College of Plant Sciences and Technology, Huazhong Agricultural University, Wuhan China
Online publish date: 2018-08-06
Publish date: 2018-11-20
Submission date: 2017-05-18
Final revision date: 2017-07-30
Acceptance date: 2017-07-31
 
Pol. J. Environ. Stud. 2019;28(1):425–433
KEYWORDS:
TOPICS:
ABSTRACT:
During our current study we evaluated the effect of ethylenediaminetetracetic acid (EDTA) and Enterobacter sp. CS2 on nickel stress alleviation and phytoextraction by Impatiens balsamina L in spiked soil. Nickel resistant Enterobacter sp. CS2 was isolated from soil polluted by industrial effluents. The I. balsamina seeds primed with Enterobacter sp. CS2 were raised in EDTA-supplemented soil (10 mM) contaminated with 0, 100, 200, and 300 mg kg-1 Ni for 50 days. The effect of different treatments on plant growth attributes, nickel tolerance index, bioconcentration factor, and translocation factor were evaluated. The Ni stress reduced plant growth, carotenoids, and chlorophyll (chl) content. However, higher Ni uptake and proline contents were observed in plants growing in Ni-contaminated soils. The Enterobacter sp. CS2 inoculation further enhanced Ni uptake and proline contents in I. balsamina plants growing under Ni stress. The inoculated plants showed improved shoot length, root length, carotenoid content, chl ‘a’ and ‘b’ contents, root and shoot dry weight. The Ni tolerance index in Enterobacter sp. CS2-assisted plants was much higher compared to un-inoculated ones. The inoculated plants supplemented with EDTA enhanced 39%, 34%, and 30% Ni uptake in roots respectively under 100, 200, and 300 mg kg-1 of Ni treatment as compared with un-inoculated plants. The data regarding bioconcentration factor and translocation factor showed that Ni phytoextraction capability of I. balsamina plants was significantly enhanced with the supplementation of Enterobacter sp. CS2 and EDTA.
CORRESPONDING AUTHOR:
Waheed Ullah Khan   
University of the Punjab, New Campus Lahore, Pakistan, 54590 Lahore, Pakistan
eISSN:2083-5906
ISSN:1230-1485