Endoglucanase and Xylanase Production by Chryseobacterium Species Isolated from Decaying Biomass
Asisipho Nkohla1, 2, Kunle Okaiyeto1, 2, Uchechukwu U. Nwodo1, 2, Leonard V. Mabinya1, 2, Anthony I. Okoh1, 2
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1SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice 5700, South Africa
2Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry
and Microbiology, University of Fort Hare, Alice 5700, South Africa
Submission date: 2016-11-07
Final revision date: 2016-12-21
Acceptance date: 2017-02-13
Online publication date: 2017-11-03
Publication date: 2017-11-07
Pol. J. Environ. Stud. 2017;26(6):2651–2660
Lignocellulosic materials are potential sources of isolating bacteria that can be used to produce important value-added products such as cellulase (endoglucanase) and xylanase in industry. Based on the above-mentioned premise, this study aimed to assess endoglucanase and xylanase-degrading potentials of a bacterial isolate from decaying sawdust samples collected from a wood factory at Melani village, Nkonkobe Municipality of Eastern Cape Province, South Africa. The bacteria showed high activity for endoglucanase and xylanase when grown on carboxymethyl cellulose (CMC) and birch wood xylan as sole carbon sources, respectively. The bacterial isolate was identified through 16S rDNA sequencing and the gene sequence was found to have 98% similarity with that of Chryseobacterium taichungense. The sequence was deposited in the GenBank as Chrysobacterium taichungense SAMRC-UFH2 with accession number KU171370. Optimum culture conditions for endoglucanase and xylanase production included: pH 6, incubation temperature (25ºC), and agitation rates of 50 rpm and 150 rpm for endoglucanase and xylanase, respectively. The high enzyme activities exhibited by this bacterial strain portend it as a potentially relevant candidate as a producer for value-added products of biotechnological importance.