Genotoxicity of Soil Pollutants Extracted with Different Solvents
Piotr Jadczyk, Barbara Kołwzan
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Institute of Environment Protection Engineering, Wrocław University of Technology,
Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland
Pol. J. Environ. Stud. 2013;22(1):141–147
The aim of our study was to compare the efficacy of extraction of genotoxic pollutants from soils performed by means of several solvents. Soil pollutants were extracted in the Soxhlet apparatus. Extract genotoxicity was assessed using the Ames Test. All the examined soils (12) contained genotoxic pollutants. Extraction with hexane allowed detection of pollution genotoxicity in 11 soils, whereas with methanol and dichloromethane in 10 soils each. The other solvents allowed detection of genotoxicity in fewer soil samples. Methanol extract had the highest genotoxicity in the majority of soils (7 out of 12). Results obtained in the present study and literature data allow recommendation of methanol for extraction of genotoxic pollutants from soils. Methanol is more versatile, efficient, common, and less harmful for laboratory workers than dichloromethane and hexane.