ORIGINAL RESEARCH
HPLC Analysis of Amoxicillin Using AccQ-Fluor
Reagent for Pre-Column Derivatization
Dorota Kowalczuk, Anna Galewska
Chair and Department of Medicinal Chemistry, Medical University of Lublin,
Chodźki 6, 20-093 Lublin, Poland
Chodźki 6, 20-093 Lublin, Poland
Pol. J. Environ. Stud. 2012;21(1):139–143
KEYWORDS
ABSTRACT
A simple procedure to perform for the determination of amoxicillin is presented, based on reaction with
1-[(quinolin-6-ylcarbamoyl)oxy]pyrrolidine-2,5-dione (AccQ-Fluor) in borate buffer medium at pH of 7.4.
The resulting amoxicillin+AccQ-Fluor derivative was determined by reversed-phase chromatography with
combined fluorescence (λex=245 nm/λem=368 nm) and UV detection (λ=245 nm) for control. The calibration
graph was linear (r=0.9992 ) over the range 5.0-30 μg/mL. The method was applied to the determination of
amoxicillin in pharmaceutical formulations with acceptable precision and accuracy.
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