Heavy Oil Biodegradation by Mixed Bacterial Consortium of Biosurfactant-Producing and Heavy Oil-Degrading Bacteria
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Beijing Key Laboratory of Remediation of Industrial Pollution Sites, Environmental Protection Research Institute of Light Industry, Beijing 10089, China
China University of Petroleum, Beijing 102249, China
Xiaoli Dai   

Environmental Protection Research Institute of Light Industry, China
Submission date: 2020-02-07
Final revision date: 2020-04-16
Acceptance date: 2020-04-18
Online publication date: 2020-09-03
Publication date: 2020-10-05
Pol. J. Environ. Stud. 2021;30(1):71–80
Environmental contamination from heavy oil is a worldwide problem. In this study, a heavy oil degrading bacterial consortium DL-1314 composed of Bacillus sp. DL-13, Brevibacillus sp. DL-1 and Acinetobacter sp. DL-34 was constructed. The constituents were all biosurfactant-producing bacteria and heavy oil-degrading bacteria. Bench-scale experiments were used to investigate the performance of the bacterial consortium in degrading heavy oil. The bacterial consortium could quickly start up the heavy oil biodegradation and degrade 60.75% of heavy oil in 8 days. Gas chromatography-mass spectrometry (GC-MS) analysis evidenced that the bacterial consortium could degrade 66.32% of saturated hydrocarbons and 63.16% of aromatic hydrocarbons, especially C15-C35 n-alkanes and 2- ring-5-ring PAHs. Analysis of the dynamic changes in the consortium DL-1314 revealed that Bacillus sp. DL-13 played a major role in the formation of surfactants in the early stage of biodegradation, Brevibacillus sp. DL-1 degraded light hydrocarbons in heavy oil, and Bacillus sp. DL-13 and Acinetobacter sp. DL-34 degraded bio-refractory hydrocarbons in heavy oil by synergistic metabolism. In-depth characterization composition of the heavy oil and the microbial consortium revealed chemical and degradation diversity, providing a more comprehensive and accurate understanding of the biodegradation process.