Influence of Aromatic, Heteroaromatic, and Alkane Hydrocarbons on the Lipase Activity of Pseudomonas sp. in Batch Culture
Elsa Cervantes-González1, Beatriz Zambrano-Monroy1, Victor Manuel Ovando-Medina1, Roberto Briones-Gallardo2, Antonio Ventura-Suarez3
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1Department of Chemistry, Academic Coordination Altiplano Region – Autonomous University of San Luis Potosi,
Road Cedral km. 5600 CP 78700, Matehuala, San Luis Potosi, Mexico
2Faculty of Engineering-Institute of Metallurgy, Autonomous University of San Luis Potosi,
Av. 550 Lomas Sierra Leone the 2nd Sec. CP 78210 San Luis Potosi, Mexico
3National School of Biological Sciences – National Polytechnic Institute,
Extending Carpio and Plan de Ayala s/n, Col. Santo Tomas Miguel Hidalgo CP 11340 Mexico, DF
Pol. J. Environ. Stud. 2014;23(5):1507–1513
The induction of the lipolytic system through the use of some hydrocarbons as the only carbon source for the growth of Pseudomonas sp. was investigated. The results showed induction of the enzymatic activity using 4 of 11 hydrocarbons used at a concentration of 3,000 mg/L (butylbenzene, 1,2,3,4-tetrahydronaphthalene, hexane and benzene), which suggests that the enzymatic expression is a function of the hydrocarbon used. Furthermore, when the concentration of these hydrocarbons was increased to 5,000 mg/L the enzymatic activity was not detected. Additionally, the effect of an external source of energy, such as sucrose, and the effect of surfactant Triton X-100 were determined. The results revealed that the presence of these may benefit the induction of the enzyme or may affect it negatively and suppress it depending of the hydrocarbon being used. Therefore, it is not feasible to consider the enzymatic activity of lipase as a biomarker in the degradation of all petroleum hydrocarbons.