Influence of Deltamethrin and Cypermethrin on the Expression of SUMO Isoforms and UBC9
Vidhya Yarahalli Jaiprakash1, Baggavalli Somesh2, Divijendra Natha Sirigiri Reddy1, Rajyalakshmi Malampati1
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1Department of Biotechnology, BMS College of Engineering, Bengaluru 560019, India
2Connexios Life Sciences Pvt Ltd, Bengaluru 560004, India
Online publish date: 2018-02-05
Publish date: 2018-03-12
Submission date: 2017-01-30
Final revision date: 2017-07-18
Acceptance date: 2017-07-18
Pol. J. Environ. Stud. 2018;27(3):1401–1406
Deltamethrin and cypermethrin are extensively used insecticides to which humans are widely exposed. The present study is designed to analyze the effects of these two pesticides on HePG2 cell lines and on differentiated 3T3-L1 and C2C12 cells with respect to the expression of small ubiquitinlike modifiers (SUMO) and SUMO-conjugating enzyme UBC9. Treatment of HePG2 cells with either deltamethrin or cypermethrin caused elevated mRNA levels of SUMO 2 and 3 as well as UBC9. This was associated with elevated nitric oxide and nitric oxide synthase mRNA levels and decreased NADPH levels. Differentiated 3T3-L1 cells treated with either deltamethrin or cypermethrin led to increased mRNA levels of inflammatory cytokines IL-Iβ and IL-6, indicating that these pesticides promote the inflammatory response. These observations are significant since previous studies reported that UBC9 and SUMO isoforms are upregulated in many types of cancers and hence the extensive use of these pesticides is a concern.