Lamium Album Extracts Express Free Radical Scavenging and Cytotoxic Activities
R. Paduch1, G. Matysik2, M. Wójciak–Kosior2, M. Kandefer–Szerszeń1, A. Skalska–Kamińska2, M. Nowak–Kryska2, P. Niedziela3
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1 Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland
2 Department of Chemistry, Laboratory of Planar Chromatography, Medical University, Staszica 6, 20-081 Lublin, Poland
3 Stationary care facility, Kolejowy Hospital, Department of Surgery, Kruczkowskiego 21, 20-468 Lublin, Poland
Pol. J. Environ. Stud. 2008;17(4):569–580
Lamium album, commonly known as “white dead nettle,” is a perennial herb widely used in folk medicine. The present paper presents the toxic, anti-proliferative, and free radical (DPPH) scavenging activities of methanol and ethyl acetate extracts of that plant. In order to determine the biologically active compounds, the plant extracts were separated by high performance thin layer chromatography (HPTLC) on silica gel Si 60 F254 and high performance liquid chromatography (HPLC) combined with densitometry. Methanol extract was rich mainly with flavonoids and phenolic acids. Ethyl acetate extract contained mainly triterpenes. Both extracts showed no toxic effects against normal human skin fibroblasts (HSF) in the range of applied concentrations (25–225 µg/ml). Anti-proliferative activity revealed that methanol extract expressed lower inhibitory properties than ethyl acetate one. The MTT test was, however, less sensitive than Neutral Red (NR) assay. Ethyl acetate extract did not exhibit DPPH radical scavenging activity. Methanol extract reduced the radical of about 29% at the highest applied concentration (225 µg/ml). Both extracts slightly influenced cellular cytoskeleton organization and amount, and size of agyrophilic nucleolar organizer regions (AgNOR) protein deposits. These findings suggest that extracts of Lamium album exhibit potential usefulness in preparation of new natural formulations.