ORIGINAL RESEARCH
Optimization of Cellulase Production
from Bacillus albus (MN755587) and Its
Involvement in Bioethanol Production
| 1 | Biology Depatrment, Faculty of Science, Jazan University, Jazan, Saudi Arabia |
| 2 | Botany and Microbiology Dep., Faculty of Science, Helwan University, Cairo, Egypt |
| 3 | Department of Biology, College of Science, University of Bisha, P.O. Box 551, Bisha 61922, Saudia Arabia |
| 4 | Biology Department, Faculty of Science, Princess Nourah bint Abdulrahman University, 84428 Riyadh, Saudi Arabia |
CORRESPONDING AUTHOR
Submission date: 2020-07-16
Final revision date: 2020-10-16
Acceptance date: 2020-11-01
Online publication date: 2021-04-01
Publication date: 2021-04-16
Pol. J. Environ. Stud. 2021;30(3):2459–2466
KEYWORDS
TOPICS
ABSTRACT
Worldwide attention has turned to utilize different cellulolytic microorganisms for the bioconversion
of cellulose into valuable products, such as bioethanol. The aim of this work is to isolate cellulaseproducing
bacteria, optimization the growth conditions, production of bioethanol and possible use in
pharmaceutical industry. Twenty cellulase-producing bacterial isolates (Coded alphabetically from A–T)
were isolated from Jazan soil. The ‘E’ isolate showed maximum cellulase production. The isolate was
characterized by 16S rRNA analysis. The gene sequence of E isolate indicated that the DNA sequence
was 99.6% homologous with 16S rRNA gene sequences of Bacillus albus. The highest cellulase activity
of B. albus was achieved when the bacterial growth media was at pH 6, supplemented with glucose,
maltose, Carboxy methyl cellulose (CMC), and lactose (1%, w/v), and inoculated with 3% (v/v) and
incubated at 35ºC for 96 h. The results indicate the liberation of 2.0 g/l reducing sugar via the DNS
method. The total reducing sugar produced was 12.4 g/l of ethanol after 72 h, when Saccharomyces
cerevisiae was used as a fermentation agent. Large scale production of bioethanol is recommended for
possible use in industry.
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