Rapid DNA Extraction for Screening Soil Filamentous Fungi Using PCR Amplification
G. A. Płaza 1*, R. Upchurch 2, R. L. Brigmon 3, W. B. Whitman 2, K. Ulfig 1
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1 Institute for Ecology of Industrial Areas, Kossutha 6, 40-844 Katowice, Poland
2 Department of Microbiology, University of Georgia, Athens, Georgia, 30602-2605, USA
3 Westinghouse Savannah River Technology Center, Aiken, South Carolina, 29808, USA
Pol. J. Environ. Stud. 2004;13(3):315–318
A simple and rapid procedure for efficiently isolating fungi DNA suitable for use as a template for PCR amplification and other molecular assays is described. The main advantages of the method are: (1) the mycelium is directly recovered from Petri-dish cultures; (2) the technique is rapid and relatively easy to perform , and (3) it allows for processing of around 50 samples during a single day; (4) it is inexpensive; (5) the quality and quantity of DNA obtained are suitable for molecular assays; (6) it can be applied to filamentous fungi from soil as well as from a fungi from other environmental sources; and (7) it does not require the use of expensive and specialized equipment or hazardous reagents.