Redox Mediators Assisted-degradation of Direct Yellow 4
Shazia Nouren1, Haq Nawaz Bhatti2, Munawar Iqbal3, Ismat Bibi4, Nosheen Nazar4, Dure Najaf Iqbal3, Qudsia Kanwal3, Abida Kausar5, Fida Hussain6
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1Department of Chemistry, Govt. College Women University, Sialkot, Pakistan
2Department of Chemistry, University of Agriculture, Faisalabad, Pakistan
3Department of Chemistry, University of Lahore, Lahore, Pakistan
4Department of Chemistry, Islamia University Bahawalpur, Pakistan
5Department of Chemistry, Government College Women University, Faisalabad, Pakistan
6Department of Botany, Qurtuba University of Science and Information,
Technology & Islamia College, Peshawar, Pakistan
Submission date: 2016-10-13
Final revision date: 2017-01-17
Acceptance date: 2017-01-18
Online publication date: 2017-10-13
Publication date: 2017-11-07
Pol. J. Environ. Stud. 2017;26(6):2885-2890
Direct yellow 4 degradation was investigated in the presence of various mediators (p-coumaric acid, 1, hydroxybenzotriazole (HOBT), syringaldehyde, vanillin, syringic acid, veratryl alcohol, and pyrocatechol) at pre-optimized conditions of process variables, i.e., pH 5.0, temperature 50ºC, enzyme dose 24 U/mL, and 0.25 mM H2O2 concentration. Citrus limon peroxidase (CL-POD) was used for degradation of DY4 dye. In the absence of mediators, the DY4 degradation was 60%, whereas mediators enhanced the POD biodegradation efficiency up to 87%. Among mediators investigated, syringaldehyde showed promising efficiency. We investigated yringaldehyde concentrations in the range of 0.0125-0.5 mM, and 0.025 mM was optimum for maximum dye degradation, which revealed that mediators could be used to enhance the biodegradation of dyes.
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