Screening and Identifying a Cadmium-resistant Fungus and Characterizing its Cadmium Adsorption
Yanan Deng1, Lifeng Wang1, Kun Luo2, Di Peng1, Huidan Jiang1,
Chenzhong Jin3, Xiaomao Zhou1, Lianyang Bai1
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1Hunan Agricultural Biotechnology Research Center, Changsha, 410125, Hunan,China
2College of Plant Protection, Hunan Agricultural University, Changsha, 410125, Hunan, China
Collaborative Innovation Center for Field Weeds Control, Loudi, 417000, Hunan, China
Submission date: 2016-10-21
Final revision date: 2016-12-08
Acceptance date: 2016-12-12
Online publication date: 2017-05-05
Publication date: 2017-05-26
Pol. J. Environ. Stud. 2017;26(3):1011–1021
The main aim of this study was to screen and identify cadmium-resistant fungus and to characterize its cadmium adsorption. A cadmium-resistant strain (CN35) was isolated from cadmium-polluted paddy soil. Based on morphlogical characteristics, internal transcribed spacers region and β-tubulin gene sequence phylogenesis analysis, the strain was preliminarily identified to be Penicillium sp. This strain was resistant to Cd at 45 mM with Cd adsorption rate up to 83.56%, and also resistant to other heavy metals such as Pb, Zn, and Cu. When Cd2+ concentration ranged from 2 to 5 mM, the fungal colony changed from yellow/green to red. The colony morphology was also affected by Cd2+ concentrations with protuberances forming on the colony surface at 20 mM. The strain CN35 was found to grow well at pH 4 to 8 at between 24ºC and 37ºC, and the optimal growth conditions were established to be at pH 4 and 30ºC. Fermented liquid of the strain is neither disease-causing nor inhibitory to rice seedling emergence, but rather improves rice seedling and root growth and enhances rice detoxification ability under Cd stress. Thus, the Cd-resistant fungus CN35 has the potential to treat Cd-polluted rice paddies.