The objective of this investigation was to assess the sanitary status of groundwater to ensure hygienic requirement water standards. Pathogens in water may originate from animals and anthropogenic waste. In a semi-rural area microorganisms are significant indicators connected with human activities in the underground water. Notably, our results showed that a more efficient diagnostic group to determine the level of contamination was proteolytic bacteria (p<0.01). Results showed that pathogenic bacteria such as Shigella sp., Salmonella sp., Escherichia coli, and total coliforms were not detected. The highest number of Staphylococcus sp. and fungal spores were found in the spring. Considerable additional research is needed to appropriately measure microbial communities in underground water.