Specific Genomic Fingerprints of Escherichia coli Strains with Repetitive Sequences and PCR as an Effective Tool For Monitoring Freshwater Environments
K. Baldy-Chudzik, M. Stosik
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Department of Microbiology and Genetics, Institute of Biotechnology and Environmental Science,
University of Zielona Góra, ul. Monte Cassino 21B, 65-561 Zielona Góra, Poland
Pol. J. Environ. Stud. 2005;14(5):551–557
The Rep-PCR fingerprinting method has been applied to identify genomic diversity of 252 E. coli strains derived in the area of a flowing-water basin. The received results show that applying UPGMA and Nearest Neighbour-Joining clustering methods to statistical analysis of rep-PCR fingerprints has made it possible to discriminate and group the strains, revealing a characteristic structure of E. coli population for particular stands of sample drawing. The proposed procedure of the analysis may be useful for routinely monitoring water quality.