The taxonomic composition of macroinvertebrates in the Liwiec River and its tributaries situated in central and eastern Poland was studied during three seasons (spring, summer and autumn). Simultaneously, physical and chemical parameters of water were measured. Water parameters were different in each study period. Macroinvertebrates samples collected in summer and autumn were much more diversified than the samples collected in spring. In the spring samples a greater EPT diversity was observed, while in the samples collected in autumn Odonata, Coleoptera and Heteroptera were more diversified. The values of the BMWP-PL index were slightly higher for the summer and autumn samples than for the spring ones. Correlation between the concentration of oxygen in water and the number of individuals of Plecoptera and Trichoptera larvae was noted. The negative correlation between the values of BOD₅, the concentration of nitrate ions and conductivity, and the number of macroinvertebrate families was observed. A negative correlation also was noted between nitrate and phosphate ion concentrations and the number of individual insect larvae.