The yeast strain Candida maltosa EH 15 was used as a biological agent in the hydrocarbon and emulsified hydrocarbon biodegradation. Six different surface active compounds were used to emulsify hydrocarbons. Biodegradation degree and biomass quantity were determined daily over 7 days. The obtained results indicated the strong ability of Candida maltosa EH 15 for hydrocarbon biodegradation. The addition of the tested surfactants influenced hydrocarbon biodegradation; however, biodegradation effectiveness varies with the type and concentration of surfactant.