The Abundance and Community Composition of Ammonia-Oxidizing Prokaryotes in Small-Reservoir Sediments in China’s Huashan Watershed
Dayong Zhao1,2, Rui Huang1,2, Jin Zeng3, Juan Luo1,2, Feng Shen1,2, Cuiling Jiang2, Feng Huang2, Zhongbo Yu1,2, Qinglong L. Wu3
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1State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University,
Nanjing 210098, China
2College of Hydrology and Water Resources, Hohai University, Nanjing 210098, China
3State Key Laboratory of Lake Science and the Environment, Nanjing Institute of Geography and Limnology,
Chinese Academy of Sciences, Nanjing 210008, China
Publish date: 2016-11-24
Submission date: 2016-03-30
Final revision date: 2016-06-20
Acceptance date: 2016-06-20
Pol. J. Environ. Stud. 2016;25(6):2665–2673
We investigated the effects of nutrient levels on the abundance and diversity of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB), seven surface sediment samples from small reservoirs at different nutrient levels were collected from the eastern, central, and western parts of Huashan watershed in Chuzhou, Anhui Province to determine the abundance and community composition of AOA and AOB. The results showed that the abundance of bacterial amoA gene (1.85×107 to 2.86×108 g/dry sediment) was higher than that of archaeal amoA gene (1.25×105 to 1.23×106 g/dry sediment) in all sediment samples. The abundance of the archaeal amoA gene exhibited significant positive correlations with total nitrogen concentrations, whereas the abundance of bacterial amoA gene showed significantly negative correlation with pH. Archaeal amoA gene sequences included Nitrososphaera and Nitrosopumilus clusters and the majority of Nitrosospira and Nitrosomonas oligotropha lineages.