The present study was conducted to assess the ability of AquaCrop model in predicting genotypes of rice genotypes grain and biological yield in water management on Rice Research Institute of Iran, for two consecutive years of 2017 and 2018. The type of experiment was split plot based on randomized complete block design with three replications per treatment. The main plot included of irrigation treatment at four levels, (flood irrigation, 5, 8 and 11 days intervals) and sub plot, included of Ali kazemi, Dorfak and Bahar rice genotypes. Evaluation simulated and measured grain yield and biological yield by adjusted coefficient of correlation and by absolute and normalized root mean square errors (RMSEn). The results indicated, the RMSEn for predicting the amounts of grain yield in validation and calibration phases for Ali kazemi, Dorfak and Bahar was assessed in the range of 6 to 8 and 8 to 9 percent respectively and the RMSEn for predicting amounts of biological yield in validation and calibration for rice genotypes was assessed in the range of 3 to 13 and 7 to 15 % respectively. The results showed that the AquaCrop model had acceptable accuracy in predicting grain yield and biological yield of the crop.