The purpose of this study was to examine the influence of environmental fluorine contamination on the adenylate nucleotides (AMP, ADP, AMP), total nucleotide pool (TAN), and adenylate energy charge (AEC ) in the erythrocytes of heifers. The concentration of F in serum as well as ATP, ADP, AMP, TAN contents and AEC of red cells were also determined. The animals exposed to F^- (group A) came from farms situated about 2-3 km away from the Police chemical plant. The control group involved animals from an ecologically clean area (group B). In comparison with the control, heifers from a study group showed a significant decrease in ATP (p≤0.001) and ADP (p≤0.01), with a parallel increase in erythrocyte AMP (p≤0.001). The means serum F- concentration was higher in exposed animals (6.5 μM, whereas in the control group it was 4.3 μM). The differences were statistically significant (p≤0.05). Both the TAN concentration in serum and the level of AEC in the group of heifers exposed to high F concentration was significantly lower than in the control group (p≤0.001).