Ultrastructural and Photosynthetic Response of Populus 107 Leaves to Cadmium Stress
Wei Ge1,2, Yunqiu Jiao1,3, Jinhua Zou1, Wusheng Jiang1, Donghua Liu1
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1Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University,
Tianjin 300387, People’s Republic of China
2Key Laboratory of Bamboo and Rattan Science and Technology of the State Forestry Administration,
International Centre for Bamboo and Rattan, Beijing 100102, People’s Republic of China
3Tianjin No. 7 Junior and Senior High School, Tianjin 300160, People’s Republic of China
Submission date: 2014-04-13
Final revision date: 2014-06-17
Acceptance date: 2014-07-08
Publication date: 2015-04-02
Pol. J. Environ. Stud. 2015;24(2):519-527
As Populus 107 is an efficient phytoextraction plant, it was used in the present investigation in order to better understand the mechanisms of detoxification and tolerance of Cd. Cd-induced impacts on photosynthetic parameters (chlorophyll content, soluble protein, and chlorophyll fluorescence) and ultrastructural changes in leaves of Populus 107 exposed to 50 μM, 100 μM, and 500 μM for 40 days were carried out. The results showed that in the Cd-treated cells, almost all the chloroplasts seemed to be affected. Cd induced several significant ultrastructural changes, including swollen chloroplast thylakoids, dissolved thylakoid grana, disintegrated chloroplasts, and numerous plastoglobuli in chloroplasts. Data from chlorophyll fluorescence showed that Fv/Fm, Fv’/Fm’, ΦPSII, ETR, and qP decreased while qN increased in leaves of Populus 107 exposed to Cd when compared to control. The content of soluble protein increased with increasing Cd concentration and declined with prolonged duration of treatment. The soluble protein content in leaves treated with 50 μM Cd reached the maximum, which was 14.29% more than that of control. The content in leaves exposed to 500 μM Cd were only 61.76% of control. At the end of the experiment the contents of chlorophyll a, b, and a+b of Populuss 107 treated with 500 μM Cd decreased to the minimum, which were 47.69%, 37.10%, and 45.49% of control, and respectively, and significantly (P < 0.05) lower than control. The toxic mechanisms of Cd are briefly explained.
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