Urban rivers were often cut off during engineering construction in regions with dense river network, causing severe deterioration of the river eco-system. Due to little research conducted on the blocked river remediation, this study applied integrated engineering measures including aeration, planted floating beds, fiber biofilm carriers, and microbial reagents to remediate a blocked river in-situ. The concentrations of NH₃-N, total phosphorus (TP), and COD_Mn of the blocked river were markedly reduced, with removal efficiencies of 50.5-94.7%, 15.8-78.2%, and 30.4-78.7% after 3-months remediation, respectively. Negative correlations were found between NH₃-N and temperature, pH, between TP and pH, probably due to season and/or pH dependent biological processes. Positive correlation between COD_Mn and dissolved oxygen suggests the efficiency of artificial aeration in COD elimination. The biofilms developed on fibers showed temporal and spatial variations in quantity and extracellular polymeric substances (EPS) composition. The biofilm EPS was dominated by humic acid-like compounds with low freshness, while the dissolved organic matter in the river mainly consisted of aromatic proteins and fulvic acid-like compounds with high freshness which may attributed to the microbial reagents. No secondary pollution from sediment was observed. Results suggest integrated ecological remediation as a feasible strategy for the blocked river remediation.