The most common indicators used for assessing combustion engines powered by renewable fuels with simultaneous fuel injection are: power, torque, fuel consumption, noise emission, amount of particulates present in the exhaust fumes and composition of exhaust fumes (oxygen, carbon dioxide, hydrocarbons, nitric oxides and air excess coefficient). The aim of this study is to analyze the impact of the addition of fatty acid methyl esters to diesel oil on the value of transport means performance parameters, particularly the level of noise generated by the transport means drive units. Generating noise by internal combustion piston engines is the effect of their operation. Our study compared emissions of sound generated by diesel oil powered with diesel oil and the mixture of 30% of fatty acid methyl esters (biocomponents) and 70% of diesel oil. The object of the study is the piston combustion engine with self-ignition of 80 kW rated power. The experiment was conducted for four scopes of the fuel controller’s settings: standard ones, increased for 2%, 4%, and 6% dosage of fuel and supercharging for 50 mb. We found that the increased dosages of fuel at powering with diesel oil without the additive of biocomponent influences the increase of the noise level within the whole tested scope.