Numerous studies concerned with accumulation of several various groups of chemical compounds have been performed on Barn Owls (Tyto alba Scop.). But up to now we have no data about concentrations of radionuclides in their tissues. However, in Europe a large part of the breeding range of this species might have received fallout from Chernobyl. Nine skeletons of Barn Owls collected in 1999-2002 in southeastern Poland were analyzed for ⁹⁰Sr, ²⁴¹Am and plutonium. The results revealed ^239+240Pu (< 0.004 Bq/kg -0.051± 0.007 Bq/kg ash weight), ²⁴¹Am (< 0.02 Bq/kg – < 0.06 Bq/kg ash weight) and ⁹⁰Sr (9.7 ± 0.9 Bq/kg – 46.8 ± 3.6 Bq/kg ash weight) concentrations to be similar to those observed in the prey species; therefore, the conclusion is that no accumulative effect has been seen.