ORIGINAL RESEARCH
Biodegradation of PAHs by Trametes hirsuta zlh237 and Effect of Bioaugmentation on PAHs-Contaminated Soil
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Lei Li 1
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Zhimin Du 1,2
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1
Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences, Zhengzhou 450008, People’s Republic of China
2
School of Environmental Engineering, Henan University of Technology, Zhengzhou, 450001, China
CORRESPONDING AUTHOR
Zhimin Du   

Institute of Biology at Henan Academy of Sciences, China
Submission date: 2021-04-20
Final revision date: 2021-09-02
Acceptance date: 2021-10-11
Online publication date: 2022-02-17
Publication date: 2022-04-06
 
Pol. J. Environ. Stud. 2022;31(3):2473–2484
 
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ABSTRACT
This study aimed to investigate the biodegradation of three distinct PAHs: Phenanthrene (Phe), Pyrene (Pyr), and Benzo[a]pyrene (BaP) by using fermentation broth from T. hirsuta culture medium. The results revealed that 2,5-xylidine ameliorates laccase production in T. hirsuta fermentation medium. After 6 days of incubation, T. hirsuta zlh237 fermentation broth (6-day-FFB) showed a higher degradation rate for three PAHs than 11-day fermentation broth (11-day-FFB). Final Phe, Pyr, and BaP degradation rates of 6-day-FFB were 89.35%, 85.92%, and 89.45%, respectively, on the 5th day of incubation. The PAHs biodegradation rate of 6-day-FFB in natural soil samples were lower than in sterilized soil. The Phe, Pyr, and BaP degradation rates of 6-day-FFB were 54.49%, 46.76%, and 51.93% in sterilized soil samples, and 39.37%, 34.00%, 33.99% in natural soil samples, after 15 days incubation, respectively. The high-throughput sequencing analysis revealed that the 6-day- FFB altered the bacterial community structure and enhanced microbial biodiversity of contaminated soil. The PAHs-contaminated soils incubated with 6-day-FFB showed significant increase in well color development than the controls. Principal Component Analysis of Biolog data differentiated the effect of 6-day-FFB and sterilized 6-day-FFB on contaminated soils. It implied that T. hirsuta zlh237 restored the microbiological functioning of the PAHs contaminated soils.
eISSN:2083-5906
ISSN:1230-1485