Biodegradation of PAHs by Trametes hirsuta zlh237 and Effect of Bioaugmentation on PAHs-Contaminated Soil
Lei Li 1
Zhimin Du 1,2
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Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences, Zhengzhou 450008, People’s Republic of China
School of Environmental Engineering, Henan University of Technology, Zhengzhou, 450001, China
Zhimin Du   

Institute of Biology at Henan Academy of Sciences, China
Submission date: 2021-04-20
Final revision date: 2021-09-02
Acceptance date: 2021-10-11
Online publication date: 2022-02-17
Publication date: 2022-04-06
Pol. J. Environ. Stud. 2022;31(3):2473–2484
This study aimed to investigate the biodegradation of three distinct PAHs: Phenanthrene (Phe), Pyrene (Pyr), and Benzo[a]pyrene (BaP) by using fermentation broth from T. hirsuta culture medium. The results revealed that 2,5-xylidine ameliorates laccase production in T. hirsuta fermentation medium. After 6 days of incubation, T. hirsuta zlh237 fermentation broth (6-day-FFB) showed a higher degradation rate for three PAHs than 11-day fermentation broth (11-day-FFB). Final Phe, Pyr, and BaP degradation rates of 6-day-FFB were 89.35%, 85.92%, and 89.45%, respectively, on the 5th day of incubation. The PAHs biodegradation rate of 6-day-FFB in natural soil samples were lower than in sterilized soil. The Phe, Pyr, and BaP degradation rates of 6-day-FFB were 54.49%, 46.76%, and 51.93% in sterilized soil samples, and 39.37%, 34.00%, 33.99% in natural soil samples, after 15 days incubation, respectively. The high-throughput sequencing analysis revealed that the 6-day- FFB altered the bacterial community structure and enhanced microbial biodiversity of contaminated soil. The PAHs-contaminated soils incubated with 6-day-FFB showed significant increase in well color development than the controls. Principal Component Analysis of Biolog data differentiated the effect of 6-day-FFB and sterilized 6-day-FFB on contaminated soils. It implied that T. hirsuta zlh237 restored the microbiological functioning of the PAHs contaminated soils.