Comparative Study of Toxicological Impinge of Glyphosate and Atrazine (Herbicide) on Stress Biomarkers; Blood Biochemical and Hematological Parameters of the Freshwater Common Carp (Cyprinus carpio)
Ahsan Khan1, Nazish Shah1, Anum Gul1, Najmu-Us-Sahar1, Afshan Ismail1, Muhammad2, Fawad Aziz1, Muhammad Farooq2, Muhammad Adnan3, Muhammad Rizwan4
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1Department of Zoology, University of Swabi, Khyber Pakhtunkhwa (KP), Pakistan
2Department of Microbiology, University of Swabi, Khyber Pakhtunkhwa (KP), Pakistan
3Department of Agriculture, University of Swabi, Khyber Pakhtunkhwa (KP), Pakistan
4Center of Biotechnology and Microbiology, University of Peshawar, Khyber Pakhtunkhwa, Pakistan
Submission date: 2016-03-30
Final revision date: 2016-04-15
Acceptance date: 2016-04-16
Publication date: 2016-10-05
Pol. J. Environ. Stud. 2016;25(5):1995–2001
The purpose of the present study was to scrutinize the effect of glyphosate and atrazine (herbicide) on blood biochemical and hematological parameters of common carp, (Cyprinus carpio), including plasma glucose (RBS), cholesterol (CH), serum protein (SP), creatinine phosphates (CPK), lactate dehydrogenase (LDH), WBC, hemoglobin (Hb), platelets (PT), lymphocytes (LP), monocytes (MT), esinophils (EN), and neutrophils (NT), and on behavioral aspects for (24, 48, 72, 96) hours under doses of (0.1, 0.07, 0.05, 0.02) and (0.2, 0.15, 0.1, 0.05)mlL-1 respectively of glyphosate and atrazine. For analysis of biochemical and hematological parameters, the protocol of biochemical analyzer set (Merck Micro Lab 300 biochemistry analyzer) and hematological analyzer (Mindray BC-2300 Hematology Analyzer) was followed in the laboratory. An upturn in RBS, CH, and WBC concentration was observed while SP, LDH, LT, MT, and EP concentrations were decreased against both herbicides. CPK and Hb concentrations were increased against atrazine, while against glyphosate the concentrations were decreased. PT and NT showed momentous upturn in concentrations against glyphosate, while showing a decline against atrazine. Both herbicides affected the blood biochemical and hematological parameters of the selected fish. Behaviorally, changes were observed against both herbicides, including loss of equilibrium, increase in the frequency of opercular movements, fast swimming and jumping, losing balance, becoming exhausted and lethargic, vertical swimming, and bleeding at the base of the eyeballs.