ORIGINAL RESEARCH
Differential Physiological and Ultrastructural Responses of Cottonseeds under Pb Toxicity
Mumtaz Khan1,2, Muhammad Daud Khan1,2, Basharat Ali1, Noor Muhammad2, Shui-Jin Zhu1
 
More details
Hide details
1Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China
2Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Pakistan
Publish date: 2014-12-16
Submission date: 2014-02-03
Final revision date: 2014-03-06
Acceptance date: 2014-03-28
 
Pol. J. Environ. Stud. 2014;23(6):2063–2070
KEYWORDS
TOPICS
Plants
 
ABSTRACT
Lead (Pb) is a toxic heavy metal that causes physiological and morphological changes in plants. Our current study was aimed at observing Pb-induced changes in seeds of two upland cotton varieties (TM-1 and Z- 747) that were exposed for 24 hours to various Pb concentrations (0, 50, 100, 300, and 500 μM). Seeds of TM-1 showed higher germination percentages, water uptake, and cell viability as compared to Z-747. Changes in nuclei shape, their partial disappearance, and increase in vacuole size were the initial signs of lead toxicity in Z-747, while TM-1 maintained its typical conformations. Cell wall thickening; increase in the number of mitochondria, protein, and lipid bodies; and vacuolation were more prominent in TM-1. In contrast, plasmolytic shrinkage and scattered cell organelles were the key characteristics of Z-747 at higher doses of Pb. Those observations demonstrated that seeds of TM-1 were more tolerant to Pb stress as compared to Z-747 and may be considered a preferred choice for culture on Pb-contaminated soils.
eISSN:2083-5906
ISSN:1230-1485