This work is a continuation of previous studies on the effects of fungicides on the uptake of trace elements by herbs [1, 2]. Copper, zinc and manganese content was determined in Valeriana officinalis grown in the soil free from additives and in the soil sprayed with thiuram. The content of bioavailable forms of these elements was established in pure soil and in the soil with fungicide addition. The introductory part of the work involves the analysis of the soil used for study (determination of organic matter content, pH, mechanical analysis, total content of Cu, Zn and Mn). The analysis showed that the soil used for the study was not contaminated with any of the examined elements.