Potential capability of heterotrophic bacteria for extracellular enzyme synthesis and their activity were determined in a transect from dunes to a water depth of 1 m in a sandy beach near Sopot on the southern Baltic coast. Among studied enzymes, alkaline phosphatase, esterase lipase, and leucine arylaminase were synthesized in a higher degree, whereas α-fucosidase, βglucouronidase and α-galactosidase had only low levels. No clear horizontal gradients were observed in the transect from dune to water. The enzyme activities of bacteria isolated from the surface and subsurface did not differ in their height and composition. Bacteria isolated form the sand of studied beach in different seasons, as a rule, synthesized the tested hydrolytic enzymes with similar intensity.