Evaluation of Resistance to Some Pyrethroid and Organophosphate Insecticides and Their Underlying Impact on the Activity of Esterases and Phosphatases in House Fly, Musca domestica (Diptera: Muscidae)
Kanwal Ranian 1  
,   Muhammad Kashif Zahoor 1  
,   Muhammad Asif Zahoor 2  
,   Hina Rizvi 3  
,   Azhar Rasul 1  
,   Humara Naz Majeed 4  
,   Farhat Jabeen 1  
,   Iqra Sarfraz 1  
,   Muhammad Zulhussnain 1  
,   Bushra Riaz 1  
,   Atta Ullah 1  
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Department of Zoology, Government College University Faisalabad, Pakistan
Department of Microbiology, Government College University Faisalabad, Pakistan
Department of Environmental Sciences & Engineering, Government College University Faisalabad, Pakistan
Department of Biochemistry, Government College Women University, Faisalabad, Pakistan
Muhammad Kashif Zahoor   

Department of Zoology, Government College University, Faisalabad, Department of Zoology, Government College University, Faisalabad, 38000 Faisalabad, Pakistan
Submission date: 2018-07-20
Final revision date: 2018-09-11
Acceptance date: 2018-09-23
Online publication date: 2020-07-31
Publication date: 2020-10-05
Pol. J. Environ. Stud. 2021;30(1):327–336
Resistance development against some frequently used insecticides, i.e., deltamethrin (1.5% EC), cypermethrin (10% SC), permethrin (0.5% WP) and DDVP (50% EC) was evaluated against Musca domestica L. Insecticide bioassays were carried out against susceptible and resistant strains at 2.5, 5, 10, 20 and 40 μg/μl concentrations. Mortality data was recorded after 24, 48 and 72 hours. Resistance was monitored up to three generations and the flies with higher LD50 values than the F1 generation were considered resistant. LD50 values for Permethrin increased from 58.258 μg/μl to 85.1375 μg/μl with highest resistance ratio (RR) in F1 to F3. The lowest resistance ratio was observed with DDVP. Maximum inhibition in adult emergence was observed against DDVP. The inhibitory activity of Esterases; Acetylcholine and Phosphatases; ACP, AKP was recorded. Deltamethrin inhibited the maximum activity of AChE (50%), whereas, DDVP caused maximum inhibition of acid phosphatases. The results suggested that house fly populations are more resistant to pyrethroids compared to organophosphate insecticides.