Production of Cellulases by Bacillus cellulosilyticus Using Lignocellulosic Material
Bushra Maryam 1,   Abdul Qadir 1  
,   Mariam Zameer 1  
,   Sajid Rashid Ahmad 1,   Rubina Nelofer 2  
,   Nadia Jamil 1  
,   Sameen Arzoo 1  
,   Rubab Afzaal 1  
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College of Earth and Environmental Sciences, (CEES) University of Punjab, Quaid-e-Azam campus, Lahore
PCSIR Laboratories, Lahore
Mariam Zameer   

University of the Punjab, college of earth and environmental sciences, university of the Punjab, Lahore, Pakistan, 54590 Lahore, Pakistan
Submission date: 2016-11-01
Final revision date: 2017-10-18
Acceptance date: 2017-11-28
Online publication date: 2018-06-28
Publication date: 2018-07-09
Pol. J. Environ. Stud. 2018;27(6):2659–2667
The enzyme cellulase, a multi enzyme complex made up of several proteins, catalyses the conversion of cellulose to glucose in an enzymatic hydrolysis. In this study biomass alkali-pretreatment followed by enzymatic hydrolysis was carried out using crude cellulase enzyme produced from screened bacillus strain (Bacillus cellulosilyticus) having 1,998.79 IU/ml/min CMCase and 1,621.16 IU/ml/min FPase enzyme activity. The production of cellulase enzyme using an economical medium has been a significant achievement in the field of industrial biotechnology. The maximum yield of sugars in the form of total sugars (179.84±0.2 mg/ml), reducing sugars (126.72±0.1 mg/ml), and glucose (105.40±0.1 mg/ml) was achieved at 48 h incubation time, 50ºC, 5 pH, 6% enzyme concentration, 4% substrate loading, and PEG 3350 as a surfactant.