Waste Frying Oil as Substrate for Lipase Production by Geotrichum candidum Strains
A. Rywińska1, D. Witkowska1, P. Juszczyk1, W. Rymowicz1, A. Kita2
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1Department of Biotechnology and Food Microbiology,
2Department of Food Storage and Technology, Wrocław University of Environmental and Life Sciences, Norwida 25/27, 50-375 Wrocław, Poland
Pol. J. Environ. Stud. 2008;17(6):925–931
The study was carried out on 9 yeast strains of Geotrichum candidum. The purpose of the study was to determine the ability of the yeast strains to produce extra- and intracellular lipases in shake cultures in a medium containing 2% and 3% of rapeseed oil used as a carbon source, after deep frying of Frenchfries. Biosynthesis of these enzymes in an AK-210 bioreactor at a working volume of 5 L was analyzed. Lipase activity was determined using olive oil (JLO) and glycerol tributyrate (JLT) as substrates. The results obtained in shake cultures showed that the best carbon source was the medium containing 2% of olive oil, while the best producer of extracellular lipases (198 JLZO mL-1) was the strain of G. candidum MSK3-11. Lipase synthesis in the bioreactor was begun at the exponential phase of growth. The activity of extracellular lipases amounted to 88 JLZO mL-1 at biomass yield of 14 g L-1 at a stationary phase. Irrespective of the strain and the medium, both extra- and intracellular activity was higher with the use of olive oil than with tributyrin.