Acute Cytotoxicity Evoked by Tetrabromobisphenol A in Primary Cultures of Rat Cerebellar Granule Cells Outweighs the Effects of Polychlorinated Biphenyls
Elżbieta Ziemińska1, Aleksandra Stafiej1, Beata Toczyłowska2,3, Jerzy W. Łazarewicz1
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1Laboratory of Pharmaconeurochemistry, Department of Neurochemistry,
Mossakowski Medical Research Centre, Polish Academy of Sciences,
Pawińskiego 5, 02-106 Warszawa, Poland
2Institute of Biochemistry and Biophysics, Polish Academy of Sciences,
Pawińskiego 5A, 02-106, Warszawa, Poland
3Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences,
Trojdena 4, 02-109 Warszawa, Poland
Pol. J. Environ. Stud. 2012;21(4):1079–1087
The aim of this study was to identify, among selected environmental toxins, the substance with the highest in vitro toxicity to neurons combined with the most marked induction of calcium imbalance, oxidative stress and mitochondrial dysfunction. Exposure of primary cultures of rat cerebellar granule cells for 30 min to polychlorinated biphenyls (PCBs) or brominated flame retardants (BFRs) at concentrations of 10-50 μM identified tetrabromobisphenol A as the compound with the highest toxicity. At a concentration of 25 μM, apart from the moderate activation of 45Ca uptake, this BFR induced the most pronounced increase in intracellular Ca2+ concentration, depolarization of mitochondria, and activation of ROS production.