Antioxidative Defense System in Pisum sativum Roots Exposed to Heavy Metals (Pb, Cu, Cd, Zn)
Arleta Malecka1, Aneta Piechalak1, Anna Mensinger1, Anetta Hanć2, Danuta Barałkiewicz2, Barbara Tomaszewska1
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1Department of Biochemistry, Institute of Molecular Biology and Biotechnology,
Adam Mickiewicz University, Collegium Biologicum,
Umultowska 89, 61-614 Poznań, Poland.
2Department of Trace Element Analysis by Spectroscopy Method, Faculty of Chemistry,
Adam Mickiewicz University, Grunwaldzka 6, 60-780 Poznań, Poland
Pol. J. Environ. Stud. 2012;21(6):1721–1730
Heavy metals (Cd, Pb, Cu, Zn) absorbed by roots brought about oxidative stress conditions through ROS production (O2¯˙, H2O2) for pea plants cultivated hydroponically for 96 h on a Hoagland medium with the addition of 50 μM: CdCl2, Pb(NO3)2, CuSO4, and ZnSO4. We shows, using laser ablation ICP MS, that Cd, Cu, Pb, and Zn elements are located along a cross-section through the roots of pea plants. We observed increasing activities of antioxidative enzymes (SOD, CAT, GR) in oxidative stress conditions. We have shown changes in redox state (GSH/GSSG) in pea root grown with Pb, Cu, Cd, and Zn.