Cadmium’s Effect on the Organization of Microtubular Cytoskeleton in Root Tips Cells of Salix matsudana Koidz
Jinhua Zou1, 2, Gang Wang1, Jing Ji1, Jiayue Wang2, Jie Ouyang2, Binbin Li2
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1School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, P.R. China
2Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University,
Tianjin 300387, P.R. China
Submission date: 2017-06-03
Final revision date: 2017-07-22
Acceptance date: 2017-07-23
Online publication date: 2018-01-10
Publication date: 2018-01-26
Pol. J. Environ. Stud. 2018;27(2):939-945
The toxic effects of Cd on microtubule (MT) organization in root tip cells of S. matsudana were investigated in the present study using tubulin immunolabeling and fluorescence microscopy. Cell damage and expression level of the SmTUA1 gene in the root tips were also examined by means of propidium iodide (PI) staining and quantitative real-time PCR (qRT-PCR) technology. The MT arrays were very sensitive to Cd. At interphase, under 50 μmol/L Cd treatment for 48 h, some cortical MTs were discontinuous, inducing numbers of differently sized fragments. With increased Cd concentrations and duration of treatment, peripheral MTs appeared to be broken gradually, and the degree of disorder was enhanced. Spindle fibers even formed condensed MT at 10 μmol/L Cd for 48 h. During anaphase/telophase, there was a small part of MT absent, MT fibers were stuck to each other (even forming lumps) and could not form phragmoplast at 50 μmol/L Cd for 48 h. The cell damage of S. matsudana root tips increased with enhanced Cd concentrations and prolonged treatment time. Expression level of SmTUA1 analyzed by qRT-PCR further validated the results by indirect immunofluorescence staining. The data obtained here will be very useful to understand the mechanisms of Cd-induced cell toxicity.
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