Effects of Lead Sulfate on Genetic and Epigenetic Changes, and Endogenous Hormone Levels in Corn (Zea mays L.)
Filiz Aygun Erturk1, Guleray Agar2, Esra Arslan2, Gokce Nardemir3, Murat Aydin4, M. Sinan Taspinar5
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1Department of Molecular Biology, Faculty of Science, Avrasya University, Trabzon, Turkey
2Department of Biology, Faculty of Science, Ataturk University, Erzurum 25240, Turkey
3Department of Molecular Biology and Genetics, Faculty of Science, Agri İbrahim Cecen University, Agri, Turkey
4Department of Field Crops, Faculty of Agriculture, Ataturk University, Erzurum, Turkey
5Department of Agricultural Biotechnology, Faculty of Agriculture, Ataturk University, Erzurum, Turkey
Submission date: 2014-03-23
Final revision date: 2014-05-23
Acceptance date: 2014-07-08
Publication date: 2014-12-16
Pol. J. Environ. Stud. 2014;23(6):1925–1932
In this research we aimed to evaluate DNA damage levels, DNA methylation, and protein and pytohormone level changes in corn seedlings (Zea mays L.) exposed to 5, 10, 20, and 40 mM concentrations of lead sulfate solution (PbSO4). The results showed that all concentrations of the test material caused a decreasing mitotic index, genomic template stability (GTS), and soluble protein levels, but increased RAPDs profile changes (DNA damage) and DNA hypermethylation. Moreover, in the HPLC (high-pressure liquid chromatography) analyses, it was also observed that Pb contamination caused a decrease in the growth-promoting hormones including gibberellic acid (GA), zeatin (ZA), and indole acetic acid (IAA) levels, in contrast to increasing the absisic acid (ABA) level. The results of this experiment have clearly shown that Pb has a significant impact on the epigenetic mechanisms as well as its genotoxic effects. Some of phytohormone decreases (GA, ZA and IAA) and especially increasing ABA levels under Pb stress may be a part of the defense system against stress.