Heterotrophic Nitrification and Aerobic Denitrification by Four Novel Isolated Bacteria
Xuanzhen Li1,2, Shengfa Wu1, Yangyang Shen1, Yongxia Ning3, Xiaolin Zhang3, Xiaoxue Sun4, Bao Zhang5, Jinyuan Chen1
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1College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032 Hangzhou, China
2College of Forestry, Henan Agricultural University, 450002 Zhengzhou, China
3College of Life Science, Henan Agricultural University, 450002 Zhengzhou, China
4College of Resource and Environmental Science, Henan Agricultural University, 450002 Zhengzhou, China
5College of Crop Science, Fujian Agriculture and Forestry University, 350002 Fujian, China
Submission date: 2014-11-26
Final revision date: 2015-01-21
Acceptance date: 2015-01-28
Publication date: 2015-07-27
Pol. J. Environ. Stud. 2015;24(4):1677–1682
Heterotrophic nitrifying bacteria are attracting increasing interest due to their advantages in treatment of wastewater with high nitrogen concentration. In this work, four bacterial isolates (WS-1, WS-4, WS-6, and WS-7) with the capability of simultaneous heterotrophic nitrification and aerobic denitrification from soil, sludge, and water by a fast isolation procedure were studied. Isolates WS-1, WS-6, and WS-7 were identified as Pseudomonas sp. and WS-4 as Agrobacterium sp. based on 16S rRNA gene sequence analysis. All four isolates could utilize ammonia, nitrate, and nitrite as sole nitrogen source and the metabolic characteristics were shown to be related with the genus. In addition, Agrobacterium sp. WS-4 accumulated less nitrate than the three Pseudomonas sp. strains with ammonia as the sole nitrogen source. When nitrate or nitrite was the sole nitrogen source, Agrobacterium sp. did not produce ammonia, and it consumed the least nitrogen compared with the three Pseudomonas sp. isolates. Our studies implied that Pseudomonas sp. strains had great potential in treating high nitrogen in wastewater.