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ORIGINAL RESEARCH
Isolation and Identification of Oil
Degrading Bacteria from Oil Tanker
Accident Locations in Jordan
1
Al-Ahliyya Amman University, Amman 19111, Jordan
Submission date: 2024-04-24
Final revision date: 2024-06-17
Acceptance date: 2024-12-09
Online publication date: 2025-02-25
Publication date: 2026-01-30
Corresponding author
Mohammad M. Aladwan
Allied Medical Science, Al-Ahliyya Amman University, Salt Street, 00962, Salt, Jordan
Allied Medical Science, Al-Ahliyya Amman University, Salt Street, 00962, Salt, Jordan
Pol. J. Environ. Stud. 2026;35(1):1011-1024
KEYWORDS
TOPICS
ABSTRACT
Oil-degrading bacteria were found in soil samples polluted with oil in the oil tanker accident
location and identified using biochemical tests and morphological analysis; fourteen bacterial isolates
were identified. Bacterial isolates were identified at the molecular level using a universal primer,
16S rDNA gene. For soil samples, gas chromatography was used to evaluate total petroleum
hydrocarbons. Heavy metal contamination (Cu, Cd, Mn, Zn, and Pb) was examined in all soil samples.
CFU/g of the bacterial growth count ranged from 2.49×105 to 1.87×1017. The following bacterial
genera were identified: Staphylococcus, Pseudomonas, Rhodococcus, Serratia, Bacillus, Acetobacter,
Micrococcus, and Lactobacillus. Lactobacillus casei, Micrococcus luteus, and Pseudomonas fluorescens
showed high growth rates on different types of hydrocarbons, such as toluene, naphthalene, and hexane.
The highest Fe, Cd, Pb, Mn, and Cu concentrations were found in soil sample M2B. However, M1A
has the highest Zn, Cu, and Fe concentrations. However, M1C has the lowest heavy metal concentrations.
M2B, however, has the lowest Zn content. Pseudomonas species, which exhibit the greatest
oil-degrading ability among all the isolates, may be utilized for bioremediation, as confirmed by
this study. At one accident site, a high concentration of Cu showed high toxicity and low ability
for hydrocarbon degradation in all bacterial isolates. Oil-contaminated locations might be cleaned up
both in-situ and ex-situ using the species found in this study.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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