How Salt Stress Represses the Biosynthesis of Marrubiin and Disturbs the Antioxidant Activity of Marrubium Vulgare L
Marwa Rezgui1, Nessrine Majdoub1, Sofiene Ben-Kaab2, Brahim Marzouk3, Houda Gouia1, Maria Eduarda M. Araújo4, Leila Bettaieb Ben-Kaab1
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1Faculté des Sciences de Tunis, University Tunis El Manar, 2092 Tunis, Tunisia
2Laboratoire des Plantes Aromatiques et Médicinales, Centre de Biotechnologie de Borj-Cedria (CBBC),
BP 901, 2050 Hammam-Lif, Tunisia
3Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj-Cedria,
2050, Hammam-Lif, Tunisia
4Centro de Química e Bioquímica, Departamento de Química e Bioquímica, Faculdade de Ciências,
Universidade de Lisboa, Campo Grande Ed. C8, 1749-016 Lisboa, Portugal
Submission date: 2016-06-12
Final revision date: 2016-08-21
Acceptance date: 2016-08-22
Online publication date: 2017-01-31
Publication date: 2017-01-31
Pol. J. Environ. Stud. 2017;26(1):267–277
growth, phenolic content, and antioxidant activities of horehound (Marrubium vulgare L.). The long-term salt treatment (up to 100 mM NaCl) resulted in significant (p<0.05) reduction of height, fresh weight, total chlorophyll, and total phenol contents. The negative effect of NaCl was accompanied by a significant restriction in K+, Ca2+, Fe2+, and Zn2+ ion uptake, and by an increase in Na+ ion concentrations – the effects of which were most pronounced at the highest NaCl level. The content of the main bioactive compound marrubiin decreased with increased NaCl concentrations. The antioxidant activity of the methanol extracts from untreated and salt-treated plants revealed that the extracts from a salt-treated plant with 100 mM exhibits the strongest activity in the DPPH and β-carotene bleaching assays, while it showed no reducing power. The present results suggest that salt treatment negatively affects the morphological, physiological, and biochemical traits of M. vulgare, which appears to be highly sensitive to salinity.