Fungal development in fodder substrates including maize has noxious effects: changes in technological properties, reduction of nutritive value, the development of mycosis and allergy agents, and the biosynthesis of refuse factors and zootoxic compounds called mycotoxins. The aim of this study was to evaluate the variation in concentrations of major Fusarium toxins along with the analysis of ergosterol in the grains of 16 maize cultivars of flint type (F), dent type (D), and combined flint/dent (F/D) and dent/flint type (D/F), respectively. The results showed that type of maize grain was a factor in determining the level of concentration of fumonisins (B₁, B₂, B₃), zearalenone, and moniliformin. However, there was no difference among grain type due to deoxynivalenol and ergosterol.