Many agricultural and biological waste materials cause environmental problems. To address this, and to achieve economic efficiency (growth, cell surface hydrophobicity (CSH), twitching, biofilm, and pyocyanin production), we used different organic wastes and Pseudomonas aeruginosa (P. aeruginosa). While there is biofilm formation in all organic waste assets, the highest such formation was observed in molasses and waste cheese whey (WCW). Average pyocyanin production in all organic waste except for Nutrient Broth (NB) averaged 3 μg/ml. CSH was reached only in the presence of olive waste water (OWW), molasses, and WCW. The highest twitching was achieved by adding WCW at 14 mm. We found no similar studies with P. aeruginosa in the literature. We think that this research will result in similar new studies that will reveal methods of handling these waste materials economically thus benefiting the environment.