We evaluated the response of Wumeng semi-fine wool sheep to a copper-contaminated environment and found an action plan to solve copper pollution through a grazing experiment and ammonium molybdate supplementary experiment carried out in Weining County of Guizhou Province in China. The content of heavy metal element in soil, herbage, and animal tissues was measured by atomic absorption spectrometry, and the blood physiological and biochemical indicators were determined by animalspecific automatic blood analyzer and automatic biochemical analyzer respectively. The results showed that the copper content in soil and herbage of contaminated pasture was significantly higher than that in control pasture, and the copper content in blood and liver in affected sheep was significantly higher than that in the control group. Hemoglobin (Hb), red blood cell count (RBC), hematocrit (PCV), mean corpuscular volume (MCV) contents and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities in affected sheep were significantly lower than those in control, while the activities of ceruloplasmin (CP), glutamic oxaloacetic transaminase (GOT), creatine phosphokinase (CPK) and malondialdehyde (MDA) were significantly higher. There was no significant difference in the level of mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and white blood cell count (WBC) between the affected and control sheep. After supplementation of ammonium molybdate, copper content in blood and liver decreased gradually, and the abnormal blood indexes recovered. At the end of the ammonium molybdate supplementation experiment, Wumeng semi-fine wool sheep in the drug-control group (CK group) showed hemoglobinuria, jaundice, anemia and other symptoms. Conclusion: a copper-contaminated environment seriously affected mineral metabolism and blood physiological and biochemical indicators of Wumeng semi-fine wool sheep, and we can utilize the antagonism of molybdenum and copper in the diet to achieve the goal of harmless utilization of a copper-polluted meadow.