ORIGINAL RESEARCH
Screening an Effective Aerobic Denitrification
Strain and its Performance in MBR
Jifeng Guo, Ping Guo, Simeng Li, Xiaojie Li,
Chongli Luo, Hui Wang, Qian Jiang
School of Environmental Science and Engineering, Key Laboratory of Subsurface Hydrology
and Ecology in Arid Areas of Education Ministry,
Chang'an University, Xi'an, 710064, P.R. China
and Ecology in Arid Areas of Education Ministry,
Chang'an University, Xi'an, 710064, P.R. China
Submission date: 2016-06-06
Final revision date: 2016-09-11
Acceptance date: 2016-12-04
Online publication date: 2017-04-27
Publication date: 2017-05-26
Pol. J. Environ. Stud. 2017;26(3):1089–1096
KEYWORDS
screeningaerobic denitrification strainmembrane bioreactor (MBR)trans-membrane pressure (TMP)trans-membranepressure (TMP)
TOPICS
ABSTRACT
A highly effective aerobic denitrification strain was screened from the municipal activated sludge and
its gene sequence was obtained from DNA extraction and amplified by the polymerase chain reaction (PCR)
amplification. Based on the result, the strain was preliminarily identified to be Pseudomonas by way of
comparison with the National Centre for Biotechnology Information (NCBI) Genbank. In order to get more
knowledge of the aerobic denitrification strain and its application in wastewater treatment, the strain was
added into the membrane bioreactor (MBR) to construct a denitrification MBR (dMBR) for municipal
wastewater treatment due to its high denitrification ability. The experiment results showed that nitrogen
removal efficiency was as high as 80% in dMBR and nitrogen removal efficiency was 50% in the control
MBR (cMBR), which indicated that aerobic denitrification in the MBR tank played a main role in removing
the nitrogen under aerobic conditions. The best strain dosage was 20-30% in the total activated sludge. The
dMBR also had a better membrane fouling resistant effect than cMBR. As a result, the dMBR could be an
effective method for nitrogen removal of the wastewater treatment.
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