The aim of the present study was to determine the reliability of sedimentation using the “Omeliansky formula” as an accepted method for quantifying airborne fungi, by comparing concentrations collected using sedimentation, impingement, and filtration methods. Significant correlation coefficients (P ≤ 0.01) and nonsignificant differences (P ≥ 0.01) were found between fungal concentrations using the three sampling methods. Sedimentation had a stronger correlation with filtration in comparison with the impingement method. Sedimentation may be accepted as a method for quantifying airborne fungi.